Agarose gel electrophoresis/Common issues

From OpenWetWare

< Agarose gel electrophoresis
Revision as of 15:12, 10 May 2005 by Jason R. Kelly (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

There are a number of issues which plague gels far a variety of reasons. This page will contain some of the more common problems and show examples of gel images that have those problems.

So if you get a gel with something bizarre, keep the image and post it here so that others can learn from your mistake.

Possible unexpected bands:

  1. Supercoiled
  2. Nicked
    • Does anyone have good examples of these?
  3. Primer-dimers (how long?)
  4. Empty site in BB vector (~300bp band)
Personal tools