AhmadWeek11

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Week 11 Journal Assignment (not done yet, thought it was due tonight, will finish by tonight)

Biological Terms

  1. Motif- The smallest group of atoms in a polymer that, when under the influence of a rotation-translation operator, will assemble the rest of the atoms in the chain. [1]
  2. Biogenesis-The process in which life forms arise from similar life forms; living things can only be produced by another living thing, and not by a non-living thing. [2]
  3. Batch Culture- large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions (In this case: nutrient limits, temperature) up to a certain density in a tank or airlift fermentor, harvested and processed as a batch, especially before all nutrients are used up. [3]
  4. Catabolite- Product of catabolism, the breakdown of complex molecules into simpler ones. [4]
  5. Trehalose- a crystalline disaccharide (C12H22O11) that is found in various organisms that is about half as sweet as sucrose, and is sometimes used as a sweetener in commercially prepared foods [5]
  6. Gene Regulatory Protein- Any protein that interacts with dna sequences of a gene and controls its transcription. [6]
  7. Transcriptome- the complement of mature messenger RNAs produced in a given cell in a given moment of its life. [7]
  8. Up-regulation- process that increases ligand/receptor interactions due to an increase in the number of available receptors. [8]
  9. Down-regluation- the process that decreases ligand and receptor interactions or reduces the responsiveness of a cell to a stimulus following first exposure.[9]
  10. Mannoprotein- yeast cell wall components that are proteins with large numbers of mannose groups attached; highly antigenic." [10]

Outline: Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis

  • Background Information
  • Experiment
    • Dilution Rate: 0.03/h
    • Temperature: One set at 12°C and another at 30°C
    • Yeast grown in a steady-state chemostat instead of batches that had been used in previous experiments
    • Anaerobic conditions
    • Haploid yeast
    • The two limited nutrients were glucose and ammonia each done separately at different temperatures making 4 data sets
    • Three replicas were done for each of the four conditions
    • Results were compared to results from previous batch experiments
    • In previous experiments, yeast were exposed to cold shock; in this experiment yeast were grown at a lower temperature
      • Adaptation vs. Acclimation


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