AhmadWeek3: Difference between revisions
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**After 61 mM, however, there was no significant increase in biomass | **After 61 mM, however, there was no significant increase in biomass | ||
**Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass] | **Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass] | ||
** | **At a level above 44 mM, oxygen consumption was equal to carbon dioxide production | ||
*Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased | |||
**Ketoglutarate: Decreased | |||
**Glutamate: Increased | |||
**Glutamine: Increased |
Revision as of 19:46, 30 January 2013
- Saccharomyces cerevisae, a type of yeast, uses ammonia as its primary nitrogen source
- Past research has shown that ammonia concentration affects growth
- In this paper, the researches wanted to know if ammonia flux, and not concentration, is the limiting factor
- Physiological Parameters
- S. cerevisae was first grown in cultures with different levels of ammonia, but fixed glucose concentrations
- Increases in the ammonia concentration from 29 to 61 mM showed a change in biomass from 4.9 to 8.2 g/L
- After 61 mM, however, there was no significant increase in biomass
- Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
- At a level above 44 mM, oxygen consumption was equal to carbon dioxide production
- Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased
- Ketoglutarate: Decreased
- Glutamate: Increased
- Glutamine: Increased