Alcazar

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (04:54, 11 August 2017) (view source)
 
(180 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:Alcazarlab2.jpg|900px]]
+
[[Image:Alcazarlab_logo.jpg|900px]]
<div style="padding: 40px; color: #ffffff; width: 900px">
<div style="padding: 40px; color: #ffffff; width: 900px">
<font face="calibri" style="color:#000000" font size="3">
<font face="calibri" style="color:#000000" font size="3">
 +
**** New lab webpage hosted at University of Barcelona servers***
 +
 +
Please visit: http://www.ub.edu/portal/web/dp-bsma/stress
<div id="right menu" style="width:250px; padding: 1em 2em; float:left;">
<div id="right menu" style="width:250px; padding: 1em 2em; float:left;">
{{Template:Alcazar Vertical Menu}}
{{Template:Alcazar Vertical Menu}}
</div>
</div>
 +
<font face="calibri" style="color:#000000" font size="4"><br>
 +
'''''Dear visitor,'''''<br>
 +
<br> Welcome to the Molecular Genetics of Plant Stress Tolerance Lab (Ruben Alcazar & A.F. Tiburcio Labs) ''' at the Department of Biology, Healthcare & Environment of the University of Barcelona. <br><br>
 +
We're a recently created joint research group that investigates the '''adaptation of plants to local environments'''. We focus on the evolutionary adaptation of plant populations to '''abiotic and biotic stresses''', which are major challenges for plant survival under the current climate change predictions. Derived from our research, we investigate ways for improving stress protection.<br>
 +
<font face="calibri" style="color:#000000" font size="4"><br>
 +
For a more detailed view of our research, please have a look at our [http://alcazar.openwetware.org/Research.html Research Projects] and [http://alcazar.openwetware.org/Publications.html Scientific Publications] sections. You can follow an updated list of our activities and publications in the NEWS section below. For any other enquires, do not hesitate to contact us directly.<br><br>
 +
<font face="helvetica" style="color:#000000" font size="2">
-
<font face="helvetica" style="color:#000000" font size="5">
 
-
<br>
 
-
'''''Plant-Environment Interactions and <br>Molecular Pharming'''''<br><br>
 
-
<font face="calibri" style="color:#000000" font size="4">
 
-
Our lab studies the genetic and molecular bases for the adaptation of plants to their local environments. We mainly focus on the adaptation to stress (abiotic and biotic) and how this can be translated in an efficient molecular pharming (production of pharmaceutical compounds in plants) under the current challenges of climate change.
 
 +
<br><br><br><br><br><br><br><br>
 +
 +
----
 +
<font face="helvetica" style="color:#000001" font size="3">
-
<font face="helvetica" style="color:#DBA901" font size="4">
 
<br>
<br>
-
Current projects <br><br>
+
'''1. POLYAMINE PERCEPTION AND SIGNALING'''
-
'''Disease resistance in plants ''' <br>
+
<div id="right menu" style="width:275px; padding: 1em 2em; float:left;">
-
<font face="calibri" style="color:#000000" font size="4"> <br>
+
[[Image:Polyamines.jpg|300px]]
-
We're currently studying the evolution of ''NB-LRR'' immune-receptor genes and other interacting loci that condition defense against evolving pathogens and how this derives in immune-related hybrid incompatibility, an important obstacle in plant breeding. We address these questions using molecular biology, population genetics and biochemical approaches. This research is complemented by the study of the modulation of ''NB-LRR'' signaling by temperature which is important under the current climate change predictions.  <br><br>
+
</div>
-
<font face="helvetica" style="color:#DBA901" font size="4"><br>
+
-
'''Natural variation of stress-induced metabolites''' <br>
+
-
<font face="calibri" style="color:#000000" font size="4"><br>
+
-
We study the genetic bases for the naturally occurring  variation of some stress metabolites involved in resistance traits to abiotic and biotic stresses in collaboration with different laboratories. We identify natural compounds which protect plants from stress. Through molecular approaches we improve the resistance of plants to different stresses. <br>
+
-
<font face="helvetica" style="color:#DBA901" font size="4"><br>
+
-
'''Molecular pharming''' <br>
+
-
<font face="calibri" style="color:#000000" font size="4"><br>
+
-
We produce proteins of pharmaceutical interest in plants and study ways to improve their productivity under different environments to adapt to the requirements of local climates.
+
-
<font face="helvetica" style="color:#000099" font size="3">
+
<br>
<br>
 +
<font face="calibri" style="color:#000000" font size="4">
 +
Our research laboratory has long-standing experience in the field of plant polyamines, for which we performed a number of omics approaches and established genetic evidence for their role in abiotic stress protection.
-
<font face="helvetica" style="color:#DBA901" font size="3"><br>
 
-
For a more detailed view of our research, have a look at our [http://alcazar.openwetware.org/Research.html Research Projects] and [http://alcazar.openwetware.org/Publications.html Scientific Publications] sections. <br> For general public information have a look at [http://alcazar.openwetware.org/Alcazar_Youtube.html +info]. <br><br>
+
Our previous research pointed to interactions of polyamines with hormones and other stress-related metabolites. Our current goal is to investigate how polyamines are perceived by the plant, which early signaling components are involved, and how this is shaped by the environment.
-
<font face="calibri" style="color:#DBA901" font size="4">
+
 +
 +
We’re making use of background knowledge from different disciplines to gain insight into polyamine perception and signaling, for which little is known in plants. We’re making use of genetics of natural variation, reverse genetics, molecular biology and plant-microbe interactions to unravel how polyamines are sensed and how this relates to the local microbiota.
 +
 +
 +
Our final goal is to provide novel approaches for crop protection against abiotic and biotic stresses.
 +
 +
 +
Key words: stress tolerance, drought, salinity, freezing tolerance, priming, ROS, crop protection, microbiome, rhizosphere, polyamines, natural variation, transcriptional regulation, metabolon, modulon.
 +
 +
 +
'''2. MOLECULAR EVOLUTION OF DISEASE RESISTANCE IN PLANTS''' <br>
 +
<font face="calibri" style="color:#000000" font size="4">
----
----
 +
In our lab, we make use of the extensive natural variation of [https://en.wikipedia.org/wiki/Arabidopsis_thaliana ''Arabidopsis thaliana''] populations to answer key fundamental questions:
-
<font face="helvetica" style="color:#ffcc66" font size="5">
+
* '''How plants adapt to local environments including the microbiota?'''  
-
[[Image:Announcement_alcazar.jpg |100px]] '''News and Announcements e-Board'''<br>
+
* '''How plants maintain variability of genes involved in pathogen recognition and to which extent this variability is caused by the interaction with pathogens?'''  
-
<font face="calibri" style="color:#000000" font size="3"><br>
+
* '''How the environment manipulates immune responses? How this affects the above points?'''
-
'''NEW'''
+
-
+
-
A 3-years PhD position (2015-2017) is available in our laboratory to study
+
-
Arabidopsis ''NB-LRR'' evolution and interactions with ''Hyaloperonospora arabidopsidis'' pathogens.
+
-
+
-
Interested candidates should send us their CV and two letters of reference by e-mail.
+
-
+
-
Questions should be addressed to the same e-mail address below.
+
-
+
-
Application period: 1 July - 15 September 2014
+
-
+
-
Expected starting date: January 2015
+
-
+
-
[mailto:ralcazar@ub.edu Contact]
+
 +
To address these and other questions, we (and others) developed a new model for plant immunity studies based on the use of ''Arabidopsis'' immune-related incompatible hybrids. These are hybrids obtained by crosses of natural ''Arabidopsis'' accessions that exhibit constitutive activation of defense, stunted growth and sterility in the absence of pathogen challenge. Often, such phenotypes are temperature-dependent and suppressed at high temperature.
 +
These genetic interactions might unravel molecular partners required for proper modulation of defense. Some of these cases are background-dependent and therefore, likely difficult to be observed in classical reference accessions.
-
Latest publications: <br>
 
-
Editorial article of upcoming e-book in Frontiers in Plant Science. Editors: Ruben Alcazar & A.F. Tiburcio.
 
-
[http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00319/full Plant polyamines in stress and development: an emerging area of research in plant sciences] <br>
 
-
'''The Roles of Polyamines during the lifespan of plants:from development to stress'''(2014).
+
Since some years, we're using the Ler / Kas-2 immune-related hybrid incompatibility as molecular model to answer some of the questions above. The Landsberg (from Gorzów Wielkopolski, Poland)/Kashmir-2 (from Kashmir mountains) incompatibility involves populations in Central Europe and Central Asia which enable to expand our analyses to population scales. We apply population genetics on top of our molecular and biochemical analyses.  
-
Tiburcio AF, Altabella T, Bitrián M and Alcázar R. Planta, doi: 10.1007/s00425-014-2055-9.
+
-
online first March 2014 [http://link.springer.com/article/10.1007/s00425-014-2055-9 read here]
+
 +
 +
Derived from our research, we attempt to provide new strategies for crop protection at medium to long-term.
-
 
-
<br><br>
 
----
----
-
[http://www.ub.edu/farmacia/ The Faculty] | [http://www.ub.edu The University] | [http://bkc.upc.ub.edu/ The Campus] | [http://www.ccit.ub.edu/EN/home.html Scientific and Technological Centers]
+
 
 +
 
 +
 
 +
 
 +
----
 +
<font face="helvetica" style="color:#000000" font size="3">
 +
[[Image:Announcement_alcazar.jpg |100px]] '''News and Announcements e-Board'''<br>
 +
<font face="calibri" style="color:#000000" font size="3"><br>
 +
  NEWS
 +
 +
 
 +
''' ACADEMIC PRESS RELEASES'''<br>
 +
[http://www.ub.edu/web/ub/en/menu_eines/noticies/2015/01/020.html? Alcázar Lab work highlighted by the University of Barcelona 20.01.15]<br>
 +
[http://www.ub.edu/noticies/cgi/event.pl?id=62490&noticiaub=FARMACIA Rubén Alcázar research highlighted by the Faculty of Pharmacy at UB, January 2015]<br>
 +
[http://sefv.net/files/sd_publicaciones/58.pdf Alcázar Lab work highlighted by the Bulletin of the Spanish Society of Plant Physiology, SEFV. January, 2015]<br>
 +
[http://www.mpipz.mpg.de/4042700/PM_Parker_2015 PLoS Genet 2014 highlight by Max Planck Institute, Cologne. 11.12.14.]<br>
 +
[http://www.ub.edu/web/ub/en/menu_eines/noticies/2013/11/063.html? Arabidopsis semidwarfs: the green revolution in nature] 02.12.2013. University of Barcelona.<br>
 +
[http://www.mpipz.mpg.de/5389/news_publication_619091 Hybrid plants with over-reactive immune system] 17.11.2010. Max Planck Society.
 +
 +
<br>
----
----
-
<br><br>
 
<font face="calibri" style="color:#000000" font size="2">
<font face="calibri" style="color:#000000" font size="2">
-
Full address: Facultat de Farmacia, Universitat de Barcelona, Unitat de Fisiologia Vegetal. Avda Joan XXIII 27-31, 08028 Barcelona (Spain). <br>
+
Rubén Alcázar is Ramón y Cajal Researcher at the Department of Biology, Healthcare and Environment of the [http://www.ub.edu University of Barcelona].<br>
-
Tel +34 934 024 492 Fax +34 934 029 043 <br><br>
+
----
-
<p>This wiki site is built under [http://www.openwetware.org Openwetware], an open access movement promoting the sharing of information among researchers.</p>
+
<br>'''Research in Alcázar Lab is supported by:'''
 +
 
 +
<font face="calibri" style="color:#000000" font size="2"><br>
 +
* 7th Framework Programme. Marie Curie Career Integration Grant (DISEASENVIRON, PCIG10-GA-2011-303568) of the European Union.
 +
* Ramón y Cajal Program (RYC-2011-07847) of the Ministerio de Ciencia e Innovación (Spain).
 +
* BFU2013-41337-P grant of the Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia (Ministerio de Economía y Competitividad, Spain).
 +
 
 +
----

Current revision

**** New lab webpage hosted at University of Barcelona servers***
Please visit: http://www.ub.edu/portal/web/dp-bsma/stress



Dept. Biology, Healthcare & Environment
Section of Plant Physiology
Facultat de Farmàcia, Av. Joan XXIII 27-31
08028 Barcelona, Spain

Map


Dear visitor,

Welcome to the Molecular Genetics of Plant Stress Tolerance Lab (Ruben Alcazar & A.F. Tiburcio Labs) at the Department of Biology, Healthcare & Environment of the University of Barcelona.

We're a recently created joint research group that investigates the adaptation of plants to local environments. We focus on the evolutionary adaptation of plant populations to abiotic and biotic stresses, which are major challenges for plant survival under the current climate change predictions. Derived from our research, we investigate ways for improving stress protection.

For a more detailed view of our research, please have a look at our Research Projects and Scientific Publications sections. You can follow an updated list of our activities and publications in the NEWS section below. For any other enquires, do not hesitate to contact us directly.












1. POLYAMINE PERCEPTION AND SIGNALING


Our research laboratory has long-standing experience in the field of plant polyamines, for which we performed a number of omics approaches and established genetic evidence for their role in abiotic stress protection.


Our previous research pointed to interactions of polyamines with hormones and other stress-related metabolites. Our current goal is to investigate how polyamines are perceived by the plant, which early signaling components are involved, and how this is shaped by the environment.


We’re making use of background knowledge from different disciplines to gain insight into polyamine perception and signaling, for which little is known in plants. We’re making use of genetics of natural variation, reverse genetics, molecular biology and plant-microbe interactions to unravel how polyamines are sensed and how this relates to the local microbiota.


Our final goal is to provide novel approaches for crop protection against abiotic and biotic stresses.


Key words: stress tolerance, drought, salinity, freezing tolerance, priming, ROS, crop protection, microbiome, rhizosphere, polyamines, natural variation, transcriptional regulation, metabolon, modulon.


2. MOLECULAR EVOLUTION OF DISEASE RESISTANCE IN PLANTS


In our lab, we make use of the extensive natural variation of Arabidopsis thaliana populations to answer key fundamental questions:

  • How plants adapt to local environments including the microbiota?
  • How plants maintain variability of genes involved in pathogen recognition and to which extent this variability is caused by the interaction with pathogens?
  • How the environment manipulates immune responses? How this affects the above points?


To address these and other questions, we (and others) developed a new model for plant immunity studies based on the use of Arabidopsis immune-related incompatible hybrids. These are hybrids obtained by crosses of natural Arabidopsis accessions that exhibit constitutive activation of defense, stunted growth and sterility in the absence of pathogen challenge. Often, such phenotypes are temperature-dependent and suppressed at high temperature.


These genetic interactions might unravel molecular partners required for proper modulation of defense. Some of these cases are background-dependent and therefore, likely difficult to be observed in classical reference accessions.


Since some years, we're using the Ler / Kas-2 immune-related hybrid incompatibility as molecular model to answer some of the questions above. The Landsberg (from Gorzów Wielkopolski, Poland)/Kashmir-2 (from Kashmir mountains) incompatibility involves populations in Central Europe and Central Asia which enable to expand our analyses to population scales. We apply population genetics on top of our molecular and biochemical analyses.


Derived from our research, we attempt to provide new strategies for crop protection at medium to long-term.






News and Announcements e-Board

 NEWS

ACADEMIC PRESS RELEASES

Alcázar Lab work highlighted by the University of Barcelona 20.01.15
Rubén Alcázar research highlighted by the Faculty of Pharmacy at UB, January 2015
Alcázar Lab work highlighted by the Bulletin of the Spanish Society of Plant Physiology, SEFV. January, 2015
PLoS Genet 2014 highlight by Max Planck Institute, Cologne. 11.12.14.
Arabidopsis semidwarfs: the green revolution in nature 02.12.2013. University of Barcelona.
Hybrid plants with over-reactive immune system 17.11.2010. Max Planck Society.



Rubén Alcázar is Ramón y Cajal Researcher at the Department of Biology, Healthcare and Environment of the University of Barcelona.



Research in Alcázar Lab is supported by:


  • 7th Framework Programme. Marie Curie Career Integration Grant (DISEASENVIRON, PCIG10-GA-2011-303568) of the European Union.
  • Ramón y Cajal Program (RYC-2011-07847) of the Ministerio de Ciencia e Innovación (Spain).
  • BFU2013-41337-P grant of the Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia (Ministerio de Economía y Competitividad, Spain).

Views
Personal tools