Alex A. Cardenas Week 12: Difference between revisions
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==Today== | ==Today== | ||
*Today we are looking at our data sets and seeing which ones were used in the microarray. | *Today we are looking at our data sets and seeing which ones were used in the microarray. | ||
*Going back to last weeks lab and answering questions. | *Going back to last weeks lab and answering questions. | ||
==Data Sets== | ===Master Spreadsheet=== | ||
*[[Media: | *So far master spreadsheet can be found [[Media:MasterSpreadsheet.xls | here]]. | ||
*[[Media:Alex_Robert_Zeb_MasterSpreadSheet_20111130_CA.xls|Master Spread Sheet]] | |||
===Data Sets=== | |||
*[[Media:ReadMeNew.txt | ReadMe]] | |||
*[[Media:E-BUGS-65.processed.1.zip | Processed]] | *[[Media:E-BUGS-65.processed.1.zip | Processed]] | ||
*[[Media:E-BUGS-65.raw.1.zip | Raw]] | *[[Media:E-BUGS-65.raw.1.zip | Raw]] | ||
*[[Media:E-BUGS-65.sdrf.xls | SRDF]] | *[[Media:E-BUGS-65.sdrf.xls | SRDF]] | ||
==Array Data Sets== | ===Array Data Sets=== | ||
*[[Media:A-BUGS-17.README.txt | ReadMe]] | *[[Media:A-BUGS-17.README.txt | ReadMe]] | ||
*[[Media:A-BUGS-17.adf.xls | ADF]] | *[[Media:A-BUGS-17.adf.xls | ADF]] | ||
==From Methods Section of Microarray== | ===From Methods Section of Microarray=== | ||
#What samples did they collect and use for the microarray experiment? | #What samples did they collect and use for the microarray experiment? | ||
#*Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained. | #*Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained. | ||
Line 25: | Line 32: | ||
#How many replicates did they perform of each type? | #How many replicates did they perform of each type? | ||
#*They performed 2 technical replicates of each type. | #*They performed 2 technical replicates of each type. | ||
#Arrary data file names found below | |||
#*Ranalexin (A1) - MBAD_12054.txt | |||
#*Ranalexin (A2) - MBAD_12061.txt | |||
#*MRSA-252 (A1) - MBAD_12055.txt | |||
#*MRSA-252 (A2) - MBAD_12060.txt | |||
#Spreadsheet can be found in the link above. | |||
==Links== | ==Links== |
Latest revision as of 11:08, 30 November 2011
Today
- Today we are looking at our data sets and seeing which ones were used in the microarray.
- Going back to last weeks lab and answering questions.
Master Spreadsheet
- So far master spreadsheet can be found here.
Data Sets
Array Data Sets
From Methods Section of Microarray
- What samples did they collect and use for the microarray experiment?
- Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained.
- How many microarray chips did they hybridize in the experiment?
- 2
- Which samples were paired to hybridize on the chip?
- Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
- Which was labeled red (Cy5)? Which was labeled green (Cy3)?
- Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5).
- Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
- How many replicates did they perform of each type?
- They performed 2 technical replicates of each type.
- Arrary data file names found below
- Ranalexin (A1) - MBAD_12054.txt
- Ranalexin (A2) - MBAD_12061.txt
- MRSA-252 (A1) - MBAD_12055.txt
- MRSA-252 (A2) - MBAD_12060.txt
- Spreadsheet can be found in the link above.
Links
- Alex A. Cardenas
- Week 12 Assignment
- Alex A. Cardenas Week 2
- Alex A. Cardenas Week 3
- Alex A. Cardenas Week 4
- Alex A. Cardenas Week 5
- Alex A. Cardenas Week 6
- Alex A. Cardenas Week 7
- Alex A. Cardenas Week 8
- Alex A. Cardenas Week 9
- Alex A. Cardenas Week 10
- Alex A. Cardenas Week 11
- Alex A. Cardenas Week 12
- Alex A. Cardenas Week 14
- Class Journal Week 1
- Class Journal Week 2
- Class Journal Week 3
- Class Journal Week 5
- Class Journal Week 6
- Class Journal Week 7
- Class Journal Week 8
- Class Journal Week 9
- Class Journal Week 10
- Class Journal Week 11
- Class Journal Week 12
- Class Journal Week 14