Alex A. Cardenas Week 12: Difference between revisions
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==Today== | |||
*Today we are looking at our data sets and seeing which ones were used in the microarray. | |||
*Going back to last weeks lab and answering questions. | |||
==Data Sets== | ==Data Sets== | ||
*[[Media:E-BUGS-65.README112.txt | ReadMe]] | *[[Media:E-BUGS-65.README112.txt | ReadMe]] | ||
Line 8: | Line 12: | ||
*[[Media:A-BUGS-17.README.txt | ReadMe]] | *[[Media:A-BUGS-17.README.txt | ReadMe]] | ||
*[[Media:A-BUGS-17.adf.xls | ADF]] | *[[Media:A-BUGS-17.adf.xls | ADF]] | ||
==From Methods Section of Microarray== | |||
#What samples did they collect and use for the microarray experiment? | |||
#*Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained. | |||
#How many microarray chips did they hybridize in the experiment? | |||
#*2 | |||
#Which samples were paired to hybridize on the chip? | |||
#*Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2). | |||
#Which was labeled red (Cy5)? Which was labeled green (Cy3)? | |||
#*Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5). | |||
#*Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3). | |||
#How many replicates did they perform of each type? | |||
#*They performed 2 technical replicates of each type. | |||
*Arrary data file names | |||
**Ranalexin (A1) - MBAD_12054.txt | |||
**Ranalexin (A2) - MBAD_12061.txt | |||
**MRSA-252 (A1) - MBAD_12055.txt | |||
**MRSA-252 (A2) - MBAD_12060.txt | |||
==Links== | ==Links== |
Revision as of 17:20, 16 November 2011
Today
- Today we are looking at our data sets and seeing which ones were used in the microarray.
- Going back to last weeks lab and answering questions.
Data Sets
Array Data Sets
From Methods Section of Microarray
- What samples did they collect and use for the microarray experiment?
- Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained.
- How many microarray chips did they hybridize in the experiment?
- 2
- Which samples were paired to hybridize on the chip?
- Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
- Which was labeled red (Cy5)? Which was labeled green (Cy3)?
- Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5).
- Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
- How many replicates did they perform of each type?
- They performed 2 technical replicates of each type.
- Arrary data file names
- Ranalexin (A1) - MBAD_12054.txt
- Ranalexin (A2) - MBAD_12061.txt
- MRSA-252 (A1) - MBAD_12055.txt
- MRSA-252 (A2) - MBAD_12060.txt
Links
- Alex A. Cardenas
- Week 12 Assignment
- Alex A. Cardenas Week 2
- Alex A. Cardenas Week 3
- Alex A. Cardenas Week 4
- Alex A. Cardenas Week 5
- Alex A. Cardenas Week 6
- Alex A. Cardenas Week 7
- Alex A. Cardenas Week 8
- Alex A. Cardenas Week 9
- Alex A. Cardenas Week 10
- Alex A. Cardenas Week 11
- Alex A. Cardenas Week 12
- Alex A. Cardenas Week 14
- Class Journal Week 1
- Class Journal Week 2
- Class Journal Week 3
- Class Journal Week 5
- Class Journal Week 6
- Class Journal Week 7
- Class Journal Week 8
- Class Journal Week 9
- Class Journal Week 10
- Class Journal Week 11
- Class Journal Week 12
- Class Journal Week 14