# Alex A. Cardenas Week 12

(Difference between revisions)
 Revision as of 13:04, 30 November 2011 (view source) (→Master Spreadsheet)← Previous diff Revision as of 13:07, 30 November 2011 (view source) (→Today)Next diff → Line 1: Line 1: + ===Master Spreadsheet=== + *So far master spreadsheet can be found [[Media:MasterSpreadsheet.xls | here]]. + + *[[Media:Alex_Robert_Zeb_MasterSpreadSheet_20111130_CA.xls|Master Spread Sheet]] + ==Today== ==Today== *Today we are looking at our data sets and seeing which ones were used in the microarray. *Today we are looking at our data sets and seeing which ones were used in the microarray. Line 12: Line 17: *[[Media:A-BUGS-17.README.txt | ReadMe]] *[[Media:A-BUGS-17.README.txt | ReadMe]] *[[Media:A-BUGS-17.adf.xls | ADF]] *[[Media:A-BUGS-17.adf.xls | ADF]] - - ===Master Spreadsheet=== - *So far master spreadsheet can be found [[Media:MasterSpreadsheet.xls | here]]. - - *[[Media:Alex_Robert_Zeb_MasterSpreadSheet_20111130_CA.xls|Master Spread Sheet]] ===From Methods Section of Microarray=== ===From Methods Section of Microarray===

## Contents

• So far master spreadsheet can be found here.

## Today

• Today we are looking at our data sets and seeing which ones were used in the microarray.
• Going back to last weeks lab and answering questions.

### From Methods Section of Microarray

1. What samples did they collect and use for the microarray experiment?
• Three biological replicates of control culture and ranalexin. A total of six arrays for analysis were obtained.
2. How many microarray chips did they hybridize in the experiment?
• 2
3. Which samples were paired to hybridize on the chip?
• Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
4. Which was labeled red (Cy5)? Which was labeled green (Cy3)?
• Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5).
• Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
5. How many replicates did they perform of each type?
• They performed 2 technical replicates of each type.
6. Arrary data file names found below