Alex J. George Week 13: Difference between revisions

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** Nitrogen-Limited Increased Expression- [[Media:Increasednitrogenlimited.txt]]
** Nitrogen-Limited Increased Expression- [[Media:Increasednitrogenlimited.txt]]
** Nitrogen-Limited Decreased Expression- [[Media:Decreasednitrogenlimited.txt]]
** Nitrogen-Limited Decreased Expression- [[Media:Decreasednitrogenlimited.txt]]
* Here are the top 10 Gene Ontology terms for
**Carbon-limited Decreased:
# Mitochondrial electron transport chain
# Energy Derivation by oxidation of organic compounds
# DNA Transposition
# Cellular Respiration
# Aerobic Respiration
# Amino acid permease activity
# Carbohydrate metabolism
# Cellular Carbohydrate metabolism
# ATP synthesis coupled electron transport
# Main Pathway of Carbohydrate Metabolism
** Carbon-limited Increased:
# Nucleolus
# Cytoplasm organization and biogenesis
# Ribosome biogenesis and assembly
# Ribosome biogenesis
# rRNA processing
# rRNA Metabolism
# Nuclear lumen
# Membrane enclosed lumen
# Organelle lumen
# RNA Binding
** Nitrogen-limited Decreased:
# Disaccharide metabolism
# Regulation of transcription from RNA polymerase to promoter
# Phosphotransferase activity/ Alcohol group as acceptor
# Trehalose metabolism
# Kinase Activity
# Transcription from RNA Polymerase to Promoter
# Carbohydrate metabolism
# Protein amino acid phosphorylation
# Protein Kinase activity
# Hydrolase activity/Hydrolyzing O-glycosyl compunds
** Nitrogen-limited Increased:
# Cytosolic Ribosome
# Ribosome
# Structural Constituent of Ribosome
# Cytoplasm Organization and Biogenesis
# Ribosome Biogenesis and assembly
# Cytosolic Large Ribosomal Subunit
# Ribonucleoprotein complex
# Nucleolus
# Ribosme Biogenesis
# Large ribosomal subunit

Revision as of 10:25, 20 April 2010

DNA Microarray Project

  • After struggling for several minutes with GenMAPP and our data, we finally got our data uploaded to GenMAPP
  • There were 70 errors while uploading
  • After uploading, we specified the Inreased and Decreased values for both Carbon and Nitrogen limiting cultures
    • Orange correlated to increase; Blue correlated to decreased
  • From here, we used MAPPfinder to analyze our data
  • The results of our data are found in the following .txt files:
  • Here are the top 10 Gene Ontology terms for
    • Carbon-limited Decreased:
  1. Mitochondrial electron transport chain
  2. Energy Derivation by oxidation of organic compounds
  3. DNA Transposition
  4. Cellular Respiration
  5. Aerobic Respiration
  6. Amino acid permease activity
  7. Carbohydrate metabolism
  8. Cellular Carbohydrate metabolism
  9. ATP synthesis coupled electron transport
  10. Main Pathway of Carbohydrate Metabolism
    • Carbon-limited Increased:
  1. Nucleolus
  2. Cytoplasm organization and biogenesis
  3. Ribosome biogenesis and assembly
  4. Ribosome biogenesis
  5. rRNA processing
  6. rRNA Metabolism
  7. Nuclear lumen
  8. Membrane enclosed lumen
  9. Organelle lumen
  10. RNA Binding
    • Nitrogen-limited Decreased:
  1. Disaccharide metabolism
  2. Regulation of transcription from RNA polymerase to promoter
  3. Phosphotransferase activity/ Alcohol group as acceptor
  4. Trehalose metabolism
  5. Kinase Activity
  6. Transcription from RNA Polymerase to Promoter
  7. Carbohydrate metabolism
  8. Protein amino acid phosphorylation
  9. Protein Kinase activity
  10. Hydrolase activity/Hydrolyzing O-glycosyl compunds
    • Nitrogen-limited Increased:
  1. Cytosolic Ribosome
  2. Ribosome
  3. Structural Constituent of Ribosome
  4. Cytoplasm Organization and Biogenesis
  5. Ribosome Biogenesis and assembly
  6. Cytosolic Large Ribosomal Subunit
  7. Ribonucleoprotein complex
  8. Nucleolus
  9. Ribosme Biogenesis
  10. Large ribosomal subunit
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