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Alex George

Apoitu II: Biochemistry

Specific Tasks

1. What are the differences in the amino acid sequences of the proteins produced by the alleles you define in Part I?
2. What features of the amino acid sequence make a protein pigmented?
3. What features of the amino acid sequence make a protein a particular color?
4. How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)?
5. Which proteins are found in each of the four starting organisms?
6. Using this knowledge, construct a purple protein.

Notes

Allele Protein Structures

• Green:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Tyr-Trp-Arg-Gln
• Blue:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Tyr-Cys-Arg-Gln
• Yellow:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Trp-Cys-Arg-Gln
• Red:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Phe-Cys-Arg-Gln
• White:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Val-Cys-Arg-Gln

Flowers

• Green: green/green
• Green2: Blue/Yellow
• Red: Red/nothing
• White: White/White

Allele Combination-->Color

• Blue/Red--> Purple

Phenotype ---> Genotype

• Green ---> cg/cg
• Blue ---> cb/cb
• White ---> cw/cw
• Red ---> cr/cr
• Orange ---> cr/cy
• Yellow ---> cy/cy
• Purple ---> cr/cb
• Black ---> cr/cg
• Definite Alleles: cg, cb,cw,cr,cy