Alexandra Sobeck: Difference between revisions
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==Research Interest== | ==Research Interest== | ||
We and others have shown that the upstream FA core complex is required for replication-dependent activation (monoubiquitination) of the downstream FANCD2 protein in response to DNA damaging agents that stall the replication fork. Our lab could also demonstrate that depletion of FA proteins from egg extracts results in accumulation of chromosomal DNA breaks during replicative DNA synthesis. | We and others have shown that the upstream FA core complex is required for replication-dependent activation (monoubiquitination) of the downstream FANCD2 protein in response to DNA damaging agents that stall the replication fork. Our lab could also demonstrate that depletion of FA proteins from egg extracts results in accumulation of chromosomal DNA breaks during replicative DNA synthesis. | ||
To further elucidate FA pathway function(s) in the replication-coupled DNA damage response I am interested in determining which DNA repair intermediates lead to specific recruitment and activation of the FA proteins. I am currently developing an activation assay in Xenopus egg extracts to test DNA minisubstrates for their ability to recruit and activate the FA core complex and/or FANCD2. Within the same experimental setting, I am using single-protein depletion strategies to investigate how the FA pathway is intertwined with other central DNA repair pathways in the DNA structure-specific damage response. | To further elucidate FA pathway function(s) in the replication-coupled DNA damage response I am interested in determining which DNA repair intermediates lead to specific recruitment and activation of the FA proteins. I am currently developing an activation assay in Xenopus egg extracts to test DNA minisubstrates for their ability to recruit and activate the FA core complex and/or FANCD2. Within the same experimental setting, I am using single-protein depletion strategies to investigate how the FA pathway is intertwined with other central DNA repair pathways in the DNA structure-specific damage response. | ||
==Published Articles== | |||
*'''Sobeck A*''', Stone S*, Costanzo V, de Graaf B, Reuter T, de Winter J, Wallisch M, Akkari Y, Olson S, Wang W, Joenje H, Christian JL, Lupardus PJ, Cimprich KA, Gautier J, Hoatlin ME ('''2006'''): Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks. Mol Cell Biol 26: 425-37. | |||
*Yin J*, '''Sobeck A*''', Xu C*, Meetei AR, Hoatlin M, Li L, Wang W ('''2005'''): BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity. EMBO J. 24: 1465-76. | |||
*Leveille F, Blom E, Medhurst AL, Bier P, Laghmani el H, Johnson M, Rooimans MA, '''Sobeck A''', Waisfisz Q, Arwert F, Patel KJ, Hoatlin ME, Joenje H, de Winter JP ('''2004'''): The Fanconi anemia gene product FANCF is a flexible adaptor protein. J Biol Chem. 279: 39421-30. | |||
*U. Oppitz, U. Bernthaler, D. Schindler, '''A. Sobeck''', H. Hoehn, M. Platzer, A. Rosenthal, and M Flentje ('''1999'''): Sequence Analysis of the ATM Gene in 20 Patients with RTOG grade 3 or 4 Acute and/or Late Tissue Radiation Side Effects. Int. J. Radiation Oncology Biol. Phys. 44, 981-988. | |||
*K.J. Erb, J.W. Holloway, '''A. Sobeck''', H. Moll, G. Le Gros ('''1998'''): Infection of mice with Mycobacterium bovis-Bacillus Calmette-Guerin (BCG) suppresses allergen-induced airway eosinophilia. J Exp Med 187:561-9. | |||
Return to Main Page [[Hoatlin Lab]] | |||
Revision as of 18:24, 2 July 2006
Alexandra Sobeck, Ph.D. Postdoctoral Fellow
Alex received her Ph.D. in Human Genetics at the Julius-Maximilians-University, Wuerzburg, Germany in 2002 after earning her M.S. in biochemistry from the Julius-Maximilian University in 1998. She arrived at OHSU in June 2002 and is currently funded as a postdoctoral fellow by the American Heart Association. Alex enjoys beef jerky, mosaics, tennis and working late hours.
Research Interest
We and others have shown that the upstream FA core complex is required for replication-dependent activation (monoubiquitination) of the downstream FANCD2 protein in response to DNA damaging agents that stall the replication fork. Our lab could also demonstrate that depletion of FA proteins from egg extracts results in accumulation of chromosomal DNA breaks during replicative DNA synthesis.
To further elucidate FA pathway function(s) in the replication-coupled DNA damage response I am interested in determining which DNA repair intermediates lead to specific recruitment and activation of the FA proteins. I am currently developing an activation assay in Xenopus egg extracts to test DNA minisubstrates for their ability to recruit and activate the FA core complex and/or FANCD2. Within the same experimental setting, I am using single-protein depletion strategies to investigate how the FA pathway is intertwined with other central DNA repair pathways in the DNA structure-specific damage response.
Published Articles
- Sobeck A*, Stone S*, Costanzo V, de Graaf B, Reuter T, de Winter J, Wallisch M, Akkari Y, Olson S, Wang W, Joenje H, Christian JL, Lupardus PJ, Cimprich KA, Gautier J, Hoatlin ME (2006): Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks. Mol Cell Biol 26: 425-37.
- Yin J*, Sobeck A*, Xu C*, Meetei AR, Hoatlin M, Li L, Wang W (2005): BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity. EMBO J. 24: 1465-76.
- Leveille F, Blom E, Medhurst AL, Bier P, Laghmani el H, Johnson M, Rooimans MA, Sobeck A, Waisfisz Q, Arwert F, Patel KJ, Hoatlin ME, Joenje H, de Winter JP (2004): The Fanconi anemia gene product FANCF is a flexible adaptor protein. J Biol Chem. 279: 39421-30.
- U. Oppitz, U. Bernthaler, D. Schindler, A. Sobeck, H. Hoehn, M. Platzer, A. Rosenthal, and M Flentje (1999): Sequence Analysis of the ATM Gene in 20 Patients with RTOG grade 3 or 4 Acute and/or Late Tissue Radiation Side Effects. Int. J. Radiation Oncology Biol. Phys. 44, 981-988.
- K.J. Erb, J.W. Holloway, A. Sobeck, H. Moll, G. Le Gros (1998): Infection of mice with Mycobacterium bovis-Bacillus Calmette-Guerin (BCG) suppresses allergen-induced airway eosinophilia. J Exp Med 187:561-9.
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