Alik:Protocols (Media): Difference between revisions
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==ES media== | ==ES media (mouse)== | ||
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Revision as of 11:33, 7 June 2006
3T3 media
450 mL | Regular DMEM |
50 mL (1 tube) | FBS |
5 mL | Pen-Strep |
ES media (mouse)
~425 mL (416.5 mL to be exact) | Knockout DMEM |
75 mL (2 tubes) | ES-FBS |
50uL | LIF |
1 mL | beta-mercaptoethanol |
5 mL | non-essential amino acids |
2.5 ml (1 tube) | L-glutamine |
5 mL | Pen-Strep |
EB media
(Same as ES media, only without Lif)
~425 mL (416.5 mL to be exact) | Knockout DMEM |
75 mL (2 tubes) | ES-FBS |
1 mL | beta-mercaptoethanol |
5 mL | non-essential amino acids |
2.5 ml (1 tube) | L-glutamine |
5 mL | Pen-Strep |