Alik:Protocols (Media): Difference between revisions
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{{Alik}} | |||
__toc__ | __toc__ | ||
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|50 mL (1 tube) | |50 mL (1 tube) | ||
|FBS | |FBS | ||
|- | |||
|5 mL | |||
|Pen-Strep | |||
|} | |} | ||
==ES media== | ==ES media (mouse)== | ||
{|class='wikitable' | {|class='wikitable' | ||
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|2.5 ml (1 tube) | |2.5 ml (1 tube) | ||
|L-glutamine | |L-glutamine | ||
|- | |||
|5 mL | |||
|Pen-Strep | |||
|} | |} | ||
==EB media== | ==EB media (mouse)== | ||
(Same as ES media, only without Lif) | (Same as ES media, only without Lif) | ||
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||2.5 ml (1 tube) | ||2.5 ml (1 tube) | ||
|L-glutamine | |L-glutamine | ||
|- | |||
|5 mL | |||
|Pen-Strep | |||
|} | |} | ||
Latest revision as of 11:33, 7 June 2006
3T3 media
| 450 mL | Regular DMEM |
| 50 mL (1 tube) | FBS |
| 5 mL | Pen-Strep |
ES media (mouse)
| ~425 mL (416.5 mL to be exact) | Knockout DMEM |
| 75 mL (2 tubes) | ES-FBS |
| 50uL | LIF |
| 1 mL | beta-mercaptoethanol |
| 5 mL | non-essential amino acids |
| 2.5 ml (1 tube) | L-glutamine |
| 5 mL | Pen-Strep |
EB media (mouse)
(Same as ES media, only without Lif)
| ~425 mL (416.5 mL to be exact) | Knockout DMEM |
| 75 mL (2 tubes) | ES-FBS |
| 1 mL | beta-mercaptoethanol |
| 5 mL | non-essential amino acids |
| 2.5 ml (1 tube) | L-glutamine |
| 5 mL | Pen-Strep |
