Alik:Protocols (Media): Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
No edit summary
Line 1: Line 1:
{{Alik}}
__toc__
__toc__



Revision as of 17:23, 14 April 2006

Home        Contact        Internal        Lab Members        Publications        Research        Talks       

3T3 media

450 mL Regular DMEM
50 mL (1 tube) FBS

ES media

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
50uL LIF
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine

EB media

(Same as ES media, only without Lif)

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine