Alik:Protocols (Media)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search

Revision as of 19:03, 14 April 2006

Contents


3T3 media

450 mL Regular DMEM
50 mL (1 tube) FBS

ES media

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
50uL LIF
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine

EB media

(Same as ES media, only without Lif)

~425 mL (416.5 mL to be exact) Knockout DMEM
75 mL (2 tubes) ES-FBS
1 mL beta-mercaptoethanol
5 mL non-essential amino acids
2.5 ml (1 tube) L-glutamine
Personal tools