Alik:Protocols (Other)

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FACS

  • Collect around 10^5 - 10^6 cells & rinse in PBS (20 000 cells are actually counted for the FACS)
  • Fix (using 4% paraformaldehyde) and permeabilize (with 0.2% triton) if necessary (both can be found in 15-ml tubes on our bench in 325)
  • Add 0.5mL of 1ary antibody (diluted in PBS) and incubate on ice for 20-30 min
  • Add 0.5mL of 2ary antibody (diluted in PBS) and incubate on ice for 20-30 min
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