Alm:PCR

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Revision as of 12:53, 21 September 2007 by ClarkeS (Talk | contribs)
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Standard PCR reactions

  • ~100 ng template
  • ~100 pmole each primer
  • buffer to 1X
  • polymerase
  • denature 94-95°C
  • anneal 5°C less than lowest primer hyb temp
  • extend 1’/kb to be amplified

5'-CATTAG can be useful to add to prevent exonuclease activity from degrading recognition sites

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