Alm:Plate reading: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Plate readers in: *Chisholm lab (unused by us, but used by Ben K. and others) *Endy lab (68-5XX): Endy:Victor3_plate_reader calculate evaporative loss to determine addition of water ...) |
No edit summary |
||
| Line 8: | Line 8: | ||
book on google calendar | book on google calendar | ||
Plates should be: | |||
*flat-bottom, clear bottom for optical measurement | |||
*tissue culture treated (not high or medium binding) | |||
*polystyrene sold sterile, also may wash | |||
If just measuring absorbance, then color is not important. Typically white plates are used for luminescent applications and black plates are used for fluorescent applications. | |||
Latest revision as of 12:01, 9 January 2008
Plate readers in:
- Chisholm lab (unused by us, but used by Ben K. and others)
- Endy lab (68-5XX): Endy:Victor3_plate_reader
calculate evaporative loss to determine addition of water
hold temperatures around 22°C
book on google calendar
Plates should be:
- flat-bottom, clear bottom for optical measurement
- tissue culture treated (not high or medium binding)
- polystyrene sold sterile, also may wash
If just measuring absorbance, then color is not important. Typically white plates are used for luminescent applications and black plates are used for fluorescent applications.
