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*Who has experience with this protocol?
*[[Sean Clarke]]
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  

Revision as of 14:33, 2 April 2007


Materials for 1 round

  • sucrose, 0.5M
  • SMM, divided
    • SMM + lysozyme


soft agar (0.8%) plates containing

In some cases, protoplasts were resus- pended in LB medium containing 0.5 M sucrose and spread onto soft agar plates containing LB medium and 0.5 M sucrose with autoclaved plastic spreaders. The plates were subsequently incubated at either 25 or 37 1C.

In other cases, the protoplasts were diluted into molten soft agar at 45 1C containing either LB medium and 0.5 M sucrose or M9 medium, 0.5 M sucrose, and either Leu (100 mg/ml) or Arg (100 mg/ml), depending on the nutritional requirements of the auxotrophic strains used, and then poured onto soft agar plates of the same composition.


Replace this sentence with a brief description of the protocol and its goal.


List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2


  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.


  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.


Relevant papers and books

Error fetching PMID 6947258:
Error fetching PMID 13718526:
  1. [--]
  2. Error fetching PMID 6947258: [Goldbeter-PNAS-1981]
  3. Error fetching PMID 13718526: [Jacob-JMB-1961]
  4. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed


or instead, discuss this protocol.

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