Altman:Protocols/Preparing Reagents

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=DESCRIPTION OF REAGENTS=
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==Description of reagents==
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Assay Buffer (AB)
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This contains the salts and the buffer that keep the biological samples happy.
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Contents of AB: 25 mM KCl, 25 mM Imidazole-Cl (pH 7.4), 1 mM EGTA, 4 mM MgCl2, 10 mM DTT
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AB is made using a 10x AB stock and a 100x DTT stock
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Dithiothreitol (DTT)
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C4H10O2S2
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DTT is a strong reducing reagent; we use it to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins
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DTT often crashes out of solution when it is frozen.  Take care to vortex the tube of DTT when you thaw an aliquot.
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Revision as of 23:39, 9 November 2012


Department of Physics, Willamette University

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==Description of reagents==
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