Altman:Protocols/Preparing Reagents/Making ATP stock solution: Difference between revisions
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==Making | ==Making 0.1 M ATP stock solution== | ||
1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich)to 8 mL of diwater | 1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich, MW 551.14) to 8 mL of diwater | ||
2. Adjust to pH 7.0 using 1 M KOH solution | 2. Adjust to pH 7.0 using 1 M KOH solution | ||
Latest revision as of 10:22, 12 March 2013
Department of Physics, Willamette University
Making 0.1 M ATP stock solution
1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich, MW 551.14) to 8 mL of diwater
2. Adjust to pH 7.0 using 1 M KOH solution
3. Raise to 10 mL with diwater
4. Aliquot (500 uL & 50 uL) and store in -20
