Altman:Protocols/Preparing Reagents/Making CPK: Difference between revisions
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*Creatine Phosphokinase – Calbiochem, Cat #: 238395, 5KU, store at -20°C | *Creatine Phosphokinase – Calbiochem, Cat #: 238395, 5KU, store at -20°C | ||
* Final stock (100x) should be 10 mg/mL stored in 0.1% BSA, 50% GlyOH, and AB conditions. | * Final stock (100x) should be 10 mg/mL stored in 0.1% BSA, 50% GlyOH, and AB conditions. | ||
1. Make ABSA + 50% GlyOH | 1. Make ABSA + 50% GlyOH | ||
Latest revision as of 17:58, 18 November 2012
Department of Physics, Willamette University
Making Creatine Phosphokinase (CPK)
- Creatine Phosphokinase – Calbiochem, Cat #: 238395, 5KU, store at -20°C
- Final stock (100x) should be 10 mg/mL stored in 0.1% BSA, 50% GlyOH, and AB conditions.
1. Make ABSA + 50% GlyOH
| 5 mL ABSA + GlyOH | |
|---|---|
| 10x AB stock | 500 uL |
| 10x BSA stock | 500 uL |
| 100x DTT stock | 50 uL |
| GlyOH | 2.5 mL |
| ddwater | fill to 5 mL |
2. Chill the ABSA + GlyOH at -20°C
3. Add 1.5 mL of ABSA + GlyOH to CPK container and mix
4. Aliquot 50 μL stocks on ice
5. Store at -20°C
