Altman:Protocols/Preparing Reagents/Making GB: Difference between revisions
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David Altman (talk | contribs) (New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Glucose Beta-Mercaptoethanol (G/β)== FINAL CONCENTRATION of 50x G/β stock: 22.5% (w...) |
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FINAL CONCENTRATION of 50x G/β stock: | FINAL CONCENTRATION of 50x G/β stock: | ||
22.5% (w/v) Glucose | * 22.5% (w/v) Glucose | ||
12.5% (v/v) β-mercaptoethanol | * 12.5% (v/v) β-mercaptoethanol | ||
Final concentration in | Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | ||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | |||
Glucose 0.225 g | |||
14.3 M β-mercaptoethanol 125 μL | <tr> | ||
1x AB 875 | <th><tt> </tt></th> | ||
<th><tt>1 mL G/β </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> Glucose </tt></th> | |||
<th><tt> 0.225 g </tt></th> | |||
<tr> | |||
<th><tt> 14.3 M β-mercaptoethanol </tt></th> | |||
<th><tt> 125 μL </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> 1x AB </tt></th> | |||
<th><tt> 875 uL </tt></th> | |||
</tr> | |||
</table> | |||
Latest revision as of 14:27, 17 November 2012
Department of Physics, Willamette University
Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
- 22.5% (w/v) Glucose
- 12.5% (v/v) β-mercaptoethanol
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
| 1 mL G/β | |
|---|---|
| Glucose | 0.225 g |
| 14.3 M β-mercaptoethanol | 125 μL |
| 1x AB | 875 uL |
