Altman:Protocols/Preparing Reagents/Making GB
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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Glucose Beta-Mercaptoethanol (G/β)== FINAL CONCENTRATION of 50x G/β stock: 22.5% (w...) |
Current revision (17:27, 17 November 2012) (view source) |
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FINAL CONCENTRATION of 50x G/β stock: | FINAL CONCENTRATION of 50x G/β stock: | ||
| - | 22.5% (w/v) Glucose | + | * 22.5% (w/v) Glucose |
| - | 12.5% (v/v) β-mercaptoethanol | + | * 12.5% (v/v) β-mercaptoethanol |
| - | + | ||
| - | Final concentration in | + | Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol |
| - | + | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | |
| - | Glucose 0.225 g | + | |
| - | 14.3 M β-mercaptoethanol 125 μL | + | <tr> |
| - | 1x AB 875 | + | <th><tt> </tt></th> |
| + | <th><tt>1 mL G/β </tt></th> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <th><tt> Glucose </tt></th> | ||
| + | <th><tt> 0.225 g </tt></th> | ||
| + | |||
| + | <tr> | ||
| + | <th><tt> 14.3 M β-mercaptoethanol </tt></th> | ||
| + | <th><tt> 125 μL </tt></th> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <th><tt> 1x AB </tt></th> | ||
| + | <th><tt> 875 uL </tt></th> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
Current revision
Department of Physics, Willamette University
