Altman:Protocols/Preparing Reagents/Making GB: Difference between revisions
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FINAL CONCENTRATION of 50x G/β stock: | FINAL CONCENTRATION of 50x G/β stock: | ||
* 22.5% (w/v) Glucose | |||
22.5% (w/v) Glucose | * 12.5% (v/v) β-mercaptoethanol | ||
12.5% (v/v) β-mercaptoethanol | |||
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | ||
Revision as of 14:24, 17 November 2012
Department of Physics, Willamette University
Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
- 22.5% (w/v) Glucose
- 12.5% (v/v) β-mercaptoethanol
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL 1x AB 875 μL
