Altman:Protocols/Preparing Reagents/Making GB

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Glucose Beta-Mercaptoethanol (G/β)== FINAL CONCENTRATION of 50x G/β stock: 22.5% (w...)
(Making Glucose Beta-Mercaptoethanol (G/β))
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FINAL CONCENTRATION of 50x G/β stock:
FINAL CONCENTRATION of 50x G/β stock:
 +
22.5% (w/v) Glucose
22.5% (w/v) Glucose
 +
12.5% (v/v)  β-mercaptoethanol  
12.5% (v/v)  β-mercaptoethanol  
 +
in assay buffer
in assay buffer
-
Final concentration in GO-juice: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
+
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
1 mL G/β
1 mL G/β

Revision as of 16:23, 17 November 2012


Department of Physics, Willamette University

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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

22.5% (w/v) Glucose

12.5% (v/v) β-mercaptoethanol

in assay buffer

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL

1x AB 875 μL
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