Altman:Protocols/Preparing Reagents/Making GB

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Glucose Beta-Mercaptoethanol (G/β)== FINAL CONCENTRATION of 50x G/β stock: 22.5% (w...)
Current revision (16:27, 17 November 2012) (view source)
 
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FINAL CONCENTRATION of 50x G/β stock:
FINAL CONCENTRATION of 50x G/β stock:
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22.5% (w/v) Glucose
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* 22.5% (w/v) Glucose
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12.5% (v/v)  β-mercaptoethanol  
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* 12.5% (v/v)  β-mercaptoethanol  
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in assay buffer
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-
Final concentration in GO-juice: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
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Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
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1 mL G/β
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
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Glucose 0.225 g
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14.3 M β-mercaptoethanol 125 μL
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<tr>
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1x AB 875 μL
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<th><tt> </tt></th>
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<th><tt>1 mL G/β </tt></th>
 +
</tr>
 +
 
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<tr>
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<th><tt> Glucose </tt></th>
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<th><tt> 0.225 g </tt></th>
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 +
<tr>
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<th><tt> 14.3 M β-mercaptoethanol </tt></th>
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<th><tt> 125 μL </tt></th>
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</tr>
 +
 
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<tr>
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<th><tt> 1x AB </tt></th>
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<th><tt> 875 uL </tt></th>
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</tr>
 +
 
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</table>

Current revision


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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

  • 22.5% (w/v) Glucose
  • 12.5% (v/v) β-mercaptoethanol

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

1 mL G/β
Glucose 0.225 g
14.3 M β-mercaptoethanol 125 μL
1x AB 875 uL
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