Altman:Protocols/Preparing Reagents/Making GB

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(Making Glucose Beta-Mercaptoethanol (G/β))
Current revision (16:27, 17 November 2012) (view source)
 
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FINAL CONCENTRATION of 50x G/β stock:
FINAL CONCENTRATION of 50x G/β stock:
 +
* 22.5% (w/v) Glucose
 +
* 12.5% (v/v)  β-mercaptoethanol
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22.5% (w/v) Glucose
+
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
-
12.5% (v/v)  β-mercaptoethanol
+
<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
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in assay buffer
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<tr>
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<th><tt> </tt></th>
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<th><tt>1 mL G/β </tt></th>
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</tr>
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Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
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<tr>
 +
<th><tt> Glucose </tt></th>
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<th><tt> 0.225 g </tt></th>
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<tr>
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<th><tt> 14.3 M β-mercaptoethanol </tt></th>
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<th><tt> 125 μL </tt></th>
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</tr>
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<tr>
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<th><tt> 1x AB </tt></th>
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<th><tt> 875 uL </tt></th>
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</tr>
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1 mL G/β
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</table>
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Glucose 0.225 g
+
-
14.3 M β-mercaptoethanol 125 μL
+
-
1x AB 875 μL
+

Current revision


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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

  • 22.5% (w/v) Glucose
  • 12.5% (v/v) β-mercaptoethanol

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

1 mL G/β
Glucose 0.225 g
14.3 M β-mercaptoethanol 125 μL
1x AB 875 uL
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