Altman:Protocols/Preparing Reagents/Making GB: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
David Altman (talk | contribs) No edit summary |
David Altman (talk | contribs) No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 12: | Line 12: | ||
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | ||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | |||
Glucose 0.225 g | |||
14.3 M β-mercaptoethanol 125 μL | <tr> | ||
1x AB 875 | <th><tt> </tt></th> | ||
<th><tt>1 mL G/β </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> Glucose </tt></th> | |||
<th><tt> 0.225 g </tt></th> | |||
<tr> | |||
<th><tt> 14.3 M β-mercaptoethanol </tt></th> | |||
<th><tt> 125 μL </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> 1x AB </tt></th> | |||
<th><tt> 875 uL </tt></th> | |||
</tr> | |||
</table> |
Latest revision as of 14:27, 17 November 2012
Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
- 22.5% (w/v) Glucose
- 12.5% (v/v) β-mercaptoethanol
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
1 mL G/β | |
---|---|
Glucose | 0.225 g |
14.3 M β-mercaptoethanol | 125 μL |
1x AB | 875 uL |