Altman:Protocols/Preparing Reagents/Making GB: Difference between revisions
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<th><tt> Glucose </tt></th> | <th><tt> Glucose </tt></th> | ||
<th><tt> 0.225 g <tr> | <th><tt> 0.225 g </tt></th> | ||
<tr> | |||
<th><tt> 14.3 M β-mercaptoethanol </tt></th> | <th><tt> 14.3 M β-mercaptoethanol </tt></th> | ||
<th><tt> 125 μL </tt></th> | <th><tt> 125 μL </tt></th> | ||
Latest revision as of 14:27, 17 November 2012
Department of Physics, Willamette University
Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
- 22.5% (w/v) Glucose
- 12.5% (v/v) β-mercaptoethanol
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
| 1 mL G/β | |
|---|---|
| Glucose | 0.225 g |
| 14.3 M β-mercaptoethanol | 125 μL |
| 1x AB | 875 uL |
