Altman:Protocols/Preparing Reagents/Making GB: Difference between revisions
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David Altman (talk | contribs) (New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Glucose Beta-Mercaptoethanol (G/β)== FINAL CONCENTRATION of 50x G/β stock: 22.5% (w...) |
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FINAL CONCENTRATION of 50x G/β stock: | FINAL CONCENTRATION of 50x G/β stock: | ||
22.5% (w/v) Glucose | 22.5% (w/v) Glucose | ||
12.5% (v/v) β-mercaptoethanol | 12.5% (v/v) β-mercaptoethanol | ||
in assay buffer | in assay buffer | ||
Final concentration in | Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol | ||
1 mL G/β | 1 mL G/β | ||
Revision as of 14:23, 17 November 2012
Department of Physics, Willamette University
Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
22.5% (w/v) Glucose
12.5% (v/v) β-mercaptoethanol
in assay buffer
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL 1x AB 875 μL
