Altman:Protocols/Preparing Reagents/Making GB
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Making Glucose Beta-Mercaptoethanol (G/β)
FINAL CONCENTRATION of 50x G/β stock:
- 22.5% (w/v) Glucose
- 12.5% (v/v) β-mercaptoethanol
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL 1x AB 875 μL