Altman:Protocols/Preparing Reagents/Making assay buffer: Difference between revisions
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==Making Assay Buffer== | ==Making Assay Buffer== | ||
Prepare the following beforehand: | |||
*100x DTT stock: 100 mM DTT | |||
*10x BSA stock: 10 mg/mL bovine serum albumin | |||
*10x AB stock | |||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | ||
Revision as of 18:39, 10 November 2012
Department of Physics, Willamette University
Making Assay Buffer
Prepare the following beforehand:
- 100x DTT stock: 100 mM DTT
- 10x BSA stock: 10 mg/mL bovine serum albumin
- 10x AB stock
| 1 mL Assay Buffer | 1 mL Assay Buffer + BSA | |
|---|---|---|
| 10x AB stock | 100 uL | 100 uL |
| 100x DTT stock | 10 uL | 10 uL |
| 10x BSA stock | -- | 100 uL |
| ddwater | 890 uL | 790 uL |
