Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

From OpenWetWare

< Altman:Protocols | Preparing Reagents(Difference between revisions)
Jump to: navigation, search
(New page: 1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate Recipes...)
Current revision (21:11, 18 November 2012) (view source)
 
(One intermediate revision not shown.)
Line 1: Line 1:
 +
{{Template:Altman}}
 +
 +
<div style="padding: 10px; width: 700px; border: 5px solid #B22222;">
 +
 +
==Making Nitrocellulose Coverslips==
 +
 +
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 +
2. Spread 7.5 μL on each slide
2. Spread 7.5 μL on each slide
 +
3. Allow coverslips to dry
3. Allow coverslips to dry
 +
4. Dip coverslips in 0.1% collodion in amyl acetate
4. Dip coverslips in 0.1% collodion in amyl acetate
 +
Recipes
Recipes
-
0.2% Triton X-100
 
-
2.5 mL 0.2% Triton
 
-
25% Triton X-100 (lab fridge) 20 μL
 
-
Filtered water 2.5 mL
 
-
0.1 % Collodion in Amyl Acetate
+
<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
-
20 mL 0.1% Collodion
+
 
-
2% collodion in amyl acetate 1 mL
+
<tr>
-
Amyl acetate 19 mL
+
<th><tt> </tt></th>
 +
<th><tt>2.5 mL 0.2% Triton </tt></th>
 +
</tr>
 +
 
 +
<tr>
 +
<th><tt> 25% Triton X-100 </tt></th>
 +
<th><tt> 20 μL </tt></th>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<th><tt> sterile water </tt></th>
 +
<th><tt> 2.5 mL </tt></th>
 +
</tr>
 +
 
 +
</table>
 +
 
 +
 
 +
<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
 +
 
 +
<tr>
 +
<th><tt> </tt></th>
 +
<th><tt> 20 mL 0.1% Collodion </tt></th>
 +
</tr>
 +
 
 +
<tr>
 +
<th><tt> 2% collodion in amyl acetate </tt></th>
 +
<th><tt> 1 mL </tt></th>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<th><tt> Amyl acetate </tt></th>
 +
<th><tt> 19 mL </tt></th>
 +
</tr>
 +
 
 +
</table>

Current revision


Department of Physics, Willamette University

'


Home        Contact        Lab Members        Publications        Research        Protocols        Pictures       


Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100

2. Spread 7.5 μL on each slide

3. Allow coverslips to dry

4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes

2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL
Personal tools