Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
David Altman (talk | contribs) No edit summary |
David Altman (talk | contribs) No edit summary |
||
| Line 7: | Line 7: | ||
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 | 1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 | ||
2. Spread 7.5 μL on each slide | 2. Spread 7.5 μL on each slide | ||
3. Allow coverslips to dry | 3. Allow coverslips to dry | ||
4. Dip coverslips in 0.1% collodion in amyl acetate | 4. Dip coverslips in 0.1% collodion in amyl acetate | ||
Recipes | Recipes | ||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | ||
Latest revision as of 18:11, 18 November 2012
Department of Physics, Willamette University
Making Nitrocellulose Coverslips
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
2. Spread 7.5 μL on each slide
3. Allow coverslips to dry
4. Dip coverslips in 0.1% collodion in amyl acetate
Recipes
| 2.5 mL 0.2% Triton | |
|---|---|
| 25% Triton X-100 | 20 μL |
| sterile water | 2.5 mL |
| 20 mL 0.1% Collodion | |
|---|---|
| 2% collodion in amyl acetate | 1 mL |
| Amyl acetate | 19 mL |
