Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips: Difference between revisions
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==Making Nitrocellulose Coverslips== | |||
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 | 1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 | ||
2. Spread 7.5 μL on each slide | 2. Spread 7.5 μL on each slide | ||
3. Allow coverslips to dry | 3. Allow coverslips to dry | ||
4. Dip coverslips in 0.1% collodion in amyl acetate | 4. Dip coverslips in 0.1% collodion in amyl acetate | ||
Recipes | Recipes | ||
0.1 | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | ||
2% collodion in amyl acetate 1 mL | <tr> | ||
Amyl acetate 19 mL | <th><tt> </tt></th> | ||
<th><tt>2.5 mL 0.2% Triton </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> 25% Triton X-100 </tt></th> | |||
<th><tt> 20 μL </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> sterile water </tt></th> | |||
<th><tt> 2.5 mL </tt></th> | |||
</tr> | |||
</table> | |||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | |||
<tr> | |||
<th><tt> </tt></th> | |||
<th><tt> 20 mL 0.1% Collodion </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> 2% collodion in amyl acetate </tt></th> | |||
<th><tt> 1 mL </tt></th> | |||
</tr> | |||
<tr> | |||
<th><tt> Amyl acetate </tt></th> | |||
<th><tt> 19 mL </tt></th> | |||
</tr> | |||
</table> | |||
Latest revision as of 18:11, 18 November 2012
Department of Physics, Willamette University
Making Nitrocellulose Coverslips
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
2. Spread 7.5 μL on each slide
3. Allow coverslips to dry
4. Dip coverslips in 0.1% collodion in amyl acetate
Recipes
| 2.5 mL 0.2% Triton | |
|---|---|
| 25% Triton X-100 | 20 μL |
| sterile water | 2.5 mL |
| 20 mL 0.1% Collodion | |
|---|---|
| 2% collodion in amyl acetate | 1 mL |
| Amyl acetate | 19 mL |
