Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

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(New page: 1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate Recipes...)
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==Making Nitrocellulose Coverslips==
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1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
2. Spread 7.5 μL on each slide
2. Spread 7.5 μL on each slide
3. Allow coverslips to dry
3. Allow coverslips to dry
4. Dip coverslips in 0.1% collodion in amyl acetate
4. Dip coverslips in 0.1% collodion in amyl acetate
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Recipes
Recipes
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0.2% Triton X-100
 
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2.5 mL 0.2% Triton
 
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25% Triton X-100 (lab fridge) 20 μL
 
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Filtered water 2.5 mL
 
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0.1 % Collodion in Amyl Acetate
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20 mL 0.1% Collodion
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
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2% collodion in amyl acetate 1 mL
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Amyl acetate 19 mL
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<tr>
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<th><tt> </tt></th>
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<th><tt>2.5 mL 0.2% Triton </tt></th>
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</tr>
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<tr>
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<th><tt> 25% Triton X-100 </tt></th>
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<th><tt> 20 μL </tt></th>
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</tr>
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<tr>
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<th><tt> sterile water </tt></th>
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<th><tt> 2.5 mL </tt></th>
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</tr>
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</table>
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">
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<tr>
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<th><tt> </tt></th>
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<th><tt> 20 mL 0.1% Collodion </tt></th>
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</tr>
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<tr>
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<th><tt> 2% collodion in amyl acetate </tt></th>
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<th><tt> 1 mL </tt></th>
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</tr>
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<tr>
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<th><tt> Amyl acetate </tt></th>
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<th><tt> 19 mL </tt></th>
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</tr>
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</table>

Revision as of 20:10, 18 November 2012


Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes


2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL
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