Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

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1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate

Recipes 0.2% Triton X-100 2.5 mL 0.2% Triton 25% Triton X-100 (lab fridge) 20 μL Filtered water 2.5 mL

0.1 % Collodion in Amyl Acetate 20 mL 0.1% Collodion 2% collodion in amyl acetate 1 mL Amyl acetate 19 mL

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