Altman:Protocols/Protein Purification/SF9 purification: Difference between revisions
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David Altman (talk | contribs) (New page: Prepare the following beforehand: Lysis Buffer 200 mM NaCl 4 mM MgCl2 20 mM Imidazole, pH 7.5 0.5 mM EDTA 1 mM EGTA 0.5% Igepal 7% sucrose 1 mM PMSF (0.25 M stock) 10 μg/mL Aprot...) |
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Revision as of 09:58, 19 February 2013
Prepare the following beforehand:
Lysis Buffer
200 mM NaCl 4 mM MgCl2 20 mM Imidazole, pH 7.5 0.5 mM EDTA 1 mM EGTA 0.5% Igepal 7% sucrose 1 mM PMSF (0.25 M stock) 10 μg/mL Aprotinin 10 μg/mL Leupeptin 5 mM DTT 2 mM ATP
1 mL Assay Buffer
(AB) 1 mL Assay Buffer + BSA
(ABSA) 10x AB stock 100 uL 100 uL 100x DTT stock 10 uL 10 uL 10x BSA stock -- 100 uL ddwater 890 uL 790 uL
