Altman:Protocols/Protein Purification/SF9 purification

Prepare the following beforehand:

Lysis Buffer

200 mM NaCl 4 mM MgCl2 20 mM Imidazole, pH 7.5 0.5 mM EDTA 1 mM EGTA 0.5% Igepal 7% sucrose 1 mM PMSF (0.25 M stock) 10 μg/mL Aprotinin 10 μg/mL Leupeptin 5 mM DTT 2 mM ATP

1 mL Assay Buffer

(AB) 1 mL Assay Buffer + BSA

(ABSA) 10x AB stock 100 uL 100 uL 100x DTT stock 10 uL 10 uL 10x BSA stock -- 100 uL ddwater 890 uL 790 uL