Altman:Protocols/Single Molecule Assays/gliding filament

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Making Nitrocellulose Coverslips)
Line 5: Line 5:
==Making Nitrocellulose Coverslips==
==Making Nitrocellulose Coverslips==
-
\emph{Typical flow-through volumes are 10-15 μL}
+
//Typical flow-through volumes are 10-15 μL//
1. Make a flow cell with a nitrocellulose-coated coverslip
1. Make a flow cell with a nitrocellulose-coated coverslip

Revision as of 15:48, 15 June 2013


Department of Physics, Willamette University

'


Home        Contact        Lab Members        Publications        Research        Protocols        Pictures       


Making Nitrocellulose Coverslips

//Typical flow-through volumes are 10-15 μL//

1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice


Recipes

50 uL Buffer #1
50x GOC stock 2 μL
100x CPK stock 1 μL
100x ATP stock 1 μL
AB 46 μL


50 μL Buffer #2
50x Glu/B stock 2 μL
100x PCr stock 1 μL
AB 47 μL


20 μL GO Juice
Buffer #1 10 μL
Buffer #2 10 μL


Personal tools