Altman:Protocols/Single Molecule Assays/gliding filament
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Making Nitrocellulose Coverslips
Typical flow-through volumes are 10-15 μL
1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice
Recipes
50 uL Buffer #1 | |
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50x GOC stock | 2 μL |
100x CPK stock | 1 μL |
100x ATP stock | 1 μL |
AB | 46 μL |
50 μL Buffer #2 | |
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50x Glu/B stock | 2 μL |
100x PCr stock | 1 μL |
AB | 47 μL |
20 μL GO Juice | |
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Buffer #1 | 10 μL |
Buffer #2 | 10 μL |