Altman:Protocols/TC Protocols/SF9 conditions: Difference between revisions
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David Altman (talk | contribs) (New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Maintaining SF9 cells== * Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks...) |
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==Maintaining SF9 cells== | ==Maintaining SF9 cells== | ||
* Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C | * Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm | ||
* Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL | * Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL | ||
Revision as of 18:42, 4 September 2013
Department of Physics, Willamette University
Maintaining SF9 cells
- Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm
- Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL
