Altman:Protocols/TC Protocols/Splitting ARPE-19

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Freezing down SF9 cells for long-term storage== 1. Remove the cell media from the cells 2. W...)
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Revision as of 21:50, 4 September 2013

Department of Physics, Willamette University


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Freezing down SF9 cells for long-term storage

1. Remove the cell media from the cells

2. Wash the cells with 5 mL of PBS

3. Remove PBS, and put in 5 mL of Trypsin

4. Incubate for 10 minutes at 37° and 5% CO2

5. Look at the cells using the microscope; you should see the cells detaching from the surface

6. Knock the flask to dislodge the cells

7. Put in 1 mL of FBS

8. Put the 6 mL of cells into a conical

9. Spin at 500 rpm for 3 minutes

10. Pull off the supernatant (6 mL)

11. Re-suspend in 10 mL of media
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