Department of Physics, Willamette University
Single molecule studies of Acanthamoeba myosin 1
From De La Cruz EM, Ostap EM. January 2004. Current Opinion in Cell Biology 16(1):61-7.
We are examining the force-sensitivity of Acanthamoeba myosin 1c (AM1C) activity. Class 1 myosins have been split into two subclasses. While subclass 2 myosins are hypothesized to have a force-dependent activity, subclass 1 myosins are not. Two subclass 2 myosins (Rat Myo1b (Laakso JM, Lewis JH, Shuman H, Ostap EM. Science. 2008 Jul 4;321(5885):133-6) and Mouse Myo1c (Greenberg MJ, Lin T, Goldman YE, Shuman H, Ostap EM. Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):E2433-40)) have been shown to have a high degree of force-sensitivity, but no subclass 1 myosin has yet been tested. To test the force sensitivity of AM1C, which is from subclass 1, we are using an optical trap, which allows us to apply picoNewton forces to single myosin motors.