Amine coupling method in SPR: Difference between revisions
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|protein||conditions||immobilization level, RU||citation | |||
|- | |||
|HSA (Human Serum Albumin) | |||
|(1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min<br> | |||
(2) HSA, 15 μg/mL same buffer 7 min<br> | |||
(3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters)<br> | |||
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein) | |||
|9,500-11,000 | |||
|<cite>karlsson1</cite> | |||
|} | |||
<biblio> | |||
#karlsson1 pmid=10821711 | |||
</biblio> | |||
[[Category:SPR]] | [[Category:SPR]] | ||
Latest revision as of 14:25, 7 April 2008
| protein | conditions | immobilization level, RU | citation |
| HSA (Human Serum Albumin) | (1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min (2) HSA, 15 μg/mL same buffer 7 min |
9,500-11,000 | [1] |
- Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. J Med Chem. 2000 May 18;43(10):1986-92. DOI:10.1021/jm991174y |
