Amine coupling method in SPR

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Current revision (17:25, 7 April 2008) (view source)
 
Line 1: Line 1:
{|border=1
{|border=1
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|protein||conditions||immobilization level||citation
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|protein||conditions||immobilization level, RU||citation
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|-
|HSA (Human Serum Albumin)
|HSA (Human Serum Albumin)
Line 7: Line 7:
(3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters)<br>
(3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters)<br>
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein)
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein)
-
|<cite>karlsson1</cite>
 
|9,500-11,000
|9,500-11,000
 +
|<cite>karlsson1</cite>
|}
|}

Current revision

proteinconditionsimmobilization level, RUcitation
HSA (Human Serum Albumin) (1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min

(2) HSA, 15 μg/mL same buffer 7 min
(3) 1M ethanolamine, pH 8.5, 7 min (block unreacted active esters)
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein)

9,500-11,000 [1]
  1. Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. . pmid:10821711. PubMed HubMed [karlsson1]
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