Amine coupling method in SPR
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protein | conditions | immobilization level, RU | citation |
HSA (Human Serum Albumin) | (1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min (2) HSA, 15 μg/mL same buffer 7 min |
9,500-11,000 | [1] |
- Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. J Med Chem. 2000 May 18;43(10):1986-92. DOI:10.1021/jm991174y |