Ampicillin

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==Mechanism of Resistance==
==Mechanism of Resistance==
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Expression of beta-lactamase neutralizes ampicillin.  When this enzyme  
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Expression of [[β-lactamase]] neutralizes ampicillin.  When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium.  As a result non-resistant satellite colonies may form around larger resistant colonies.
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(would be nice to have a picture of this here)
==References==
==References==
Molecular Cloning: A Laboratory Manual, Vol 1.
Molecular Cloning: A Laboratory Manual, Vol 1.

Revision as of 13:08, 11 May 2005

Mode of Action

Inhibits the formation of cross-links in the peptidoglycan layer (which prvides rigidity to the cell wall). Most effctive against cells in log phase growth (since this is when new cross-links are being formed), and has little effect on cells in stationary phase.

Mechanism of Resistance

Expression of β-lactamase neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies.

(would be nice to have a picture of this here)

References

Molecular Cloning: A Laboratory Manual, Vol 1.

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