Angela A. Garibaldi Week 7
From OpenWetWare
Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human anti-body
Introduction
- HIV destroys CD4+ lymphocytes
Basic Structure
- Viral envelope (responsible for entry of HIV to host cells)has glycoproteins which contain oligomeric spikes, trimeric spikes on the surface
- gp41 (transmembrane envelope glycoprotein) anchors the spikes in the viral membrane
- spike surface made of gp120 (exterior envelope glycoprotein) which have non-covalent interactions with each subunit of trimeric gp41 complex
- 5 variable regions
- 1-4 form loops exposed to the surface which are bonded at their bases by disulfide bonds.
Basic Entry and Binding
Structure
Structure determination
Structure of gp120
Figure 1
Figure 2
Interactions
CD4-gp120 interaction
Figure 3
Interfacial Cavities
Antibody interface
Figure 4
Oligomer and gp41 interactions
Conformational change in core gp120
Figure 5
Viral evasion of immune responses
Mechanistic implications for virus entry
Methods
Protein production, crystallization and data collection
Structure determination and refinement
Table 1
Structure Analysis
Definitions
- epitope -That part of an antigenic molecule to which the T-cell receptor responds; a site on a large molecule against which an antibody will be produced and to which it will bind. biology-online.org dictionary
- chemokine -
- oligomeric spike/complex -
- trimeric spike/complex -
- fusogenic -
- anti-hotspot -
- cavity -
- linchpin -
- humoral immune response -
- oligomeric occlusion -
- glycocalyx -
