Angela A. Garibaldi Week 8
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Retrieving Protein Sequences
- Go to UniProt [www.expasy.org/sprot UniProt]
- Enter dUTPase in search window. This produces more than 3 relevant sequences, so found DUT ECOLI (P06968) on page 4
- Scroll down for FASTA format of amino acid sequences
- In the case that your beginning information is not enough to find the protein sequence you seek,
- find the advanced search option. This no longer exists. You have to click the add and search button and a drop down menu will be displayed to give you the same search options as described in Figure 2-16 of the Bioinformatics for Dummeies
Retrieving a List of Related Protein Sequences
- Go to the Advanced Search UniProt as described above
- Because the advanced search is completely different, cannot deselect TrEMBL
- Input dUTPase in search again. There is no "description" field any longer.Yields many possibilities
- Since there are more than 211 total possibilities, so we selected entire first page of sequences (25)
- In newer version click retrieve at the bottom right corner instead of french button.
- Once you retrieve these, it is put into a list of which you can add to and then below choose the format you want the sequences in. No longer have to copy and paste into a document. FASTA format is available.