Anneal Extend PCR Protocol - for linking 2 pieces of DNA: Difference between revisions
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This is a protocol for linking any 2 pieces of DNA together using homologous primers. | This is a protocol for linking any 2 pieces of DNA together using homologous primers. | ||
The original protocol can be found here[http://openwetware.org/ | The original protocol can be found here[http://openwetware.org/images/9/95/Anneal_Extend.pdf]. | ||
As well, there is a protocol for introducing mutations via the primer. That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf]. | As well, there is a protocol for introducing mutations via the primer. That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf]. |